Characterization of eugonic fermenters group EF-4 by polyacrylamide gel electrophoresis and protein immunoblot analysis.

Record Detail

Save:
Title:	Characterization of eugonic fermenters group EF-4 by polyacrylamide gel electrophoresis and protein immunoblot analysis.
Author:	Hanner, T.L. : Allen, J.W. : Robertson Byers, A. : Hurley, S.L.
Citation:	Am-J-Vet-Res. Schaumburg, Ill. : American Veterinary Medical Association. July 1991. v. 52 (7) p. 1065-1068.
Abstract:	Whole-cell lysates and proteinase K-extracted lipopolysaccharide (LPS) of 19 strains of the group eugonic fermenter-4 (EF-4) were analyzed by electrophoresis and protein immunoblotting. These strains were isolated from dog- and cat-bite abscesses in human beings, ferret and human gastric lesions, and cat-lung infections. These strains represent 2 biovar groupings; EF-4a biovars ferment glucose and possess arginine dihydrolase activity, whereas EF-4b biovars do not. Electrophoresis of whole-cell lysates could distinguish between these biovars groups. Electrophoresis of LPS extracts revealed that all strains of EF-4 possess smooth chemotypes. Two strains of EF-4a reacted weakly in protein immunoblots and revealed distinct LPS profiles. These studies suggests that subgroups of EF-4 biovars may exist.
Review References:	None
Notes:	Includes references.
Language:	English
Publication Type:	Article
Keywords:	None
URL:	No URL associated with this record.